eBook: Techniques for high-content live cell imaging

High-content images contain a wealth of data, and automated image analysis can extract hundreds of metrics from a single frame, such as cell area, dry mass, and details of organelle networks. Capturing this information through imaging can be carried out in many ways, from label-free methods to cell painting, and high-content imaging is a fast-moving, innovative field. 

Do you want to compare label-free and fluorescence microscopy methods to find the best fit for high-content analysis, but don’t know where to start? This eBook compares different live cell imaging methods and asks: 

What is live cell imaging? 

What is high-content imaging? 

Which live cell imaging methods give organelle-level resolution? 

How do different imaging methods affect cell viability? 

How can high-content images be analyzed? 

• Widefield fluorescence 

• Point scanning and spinning disk confocal 

• Light sheet microscopy (SPIM) 

What is holotomography, how does it work and what benefits does this live-cell imaging approach bring to fundamental research and drug development? This miniseries dives into the technology behind Nanolive’s high-content live cell images and explains how holotomography is essential for providing deep phenotypic insights into cellular responses.