Nanolive technology reveals ferroptosis execution in an aggressive human prostate cancer model

Identifying the morphological changes that characterize ferroptosis is imperative to understand the pervasiveness of this type of cell death. Nothing is currently known about the morphology and/or distribution of lipid droplets during ferroptosis, despite the critical importance of lipid peroxidation in this type of cell death [4]. Nanolive’s 3D Cell Explorer (see here) can image lipid droplets without the addition of labels, for long periods of time (from days to weeks) and so we set about investigating this question.

This video was collected during a demonstration conducted at the Department of Pathology, in the University of Göttingen (Germany). Assistant Professor Dr. Vivek Venkataramani is the first to apply cutting-edge Nanolive imaging technology to investigate ferroptosis. He induced ferroptosis in PC3 cells (an androgen-independent prostate cancer cell line) using RNA-interference (RNAi) with the selective glutathione peroxidase 4 inhibitor, RSL-3 and then imaged the cells over a period of 24 h, at an acquisition rate of 1 image every 10 min.