Newsletter June: From live cells to insights: Streamline immunotherapy discovery without labels

Welcome to the June edition of the AI for Live Cell Insights Newsletter, bringing you to the latest live cell analyses powering drug discovery and cosmetics development. Each month, we will explore a new application of AI-based cellular analysis for label-free live cell imaging, with publication highlights and news from Nanolive. This month, we are thrilled to announce the release of Nanolive’s next generation LIVE T Cell Assay, a cutting-edge AI-powered solution designed to redefine immunotherapy research. Keep reading to discover the assay’s key features and applications, as well as recent publications in the fields of immune-oncology and cellular metabolism.

High-content, label-free immune cell analysis with the LIVE T Cell Assay

With the LIVE T Cell Assay, you can:

• Capture dynamic T cell:target cell interactions and phenotypic changes in real-time, label-free

• Streamline experimental workflows to reduce time, cost, and variability

• Generate unbiased, reproducible data that increase confidence in drug discovery

• Apply the assay to both liquid and solid tumor models for broader relevance across cancer types

Download our recent scientific poster, Guide to label-free analysis of T cell activity and target cell killing, to explore some key readouts that you can obtain from a single experiment!

Real-world applications in CAR-T research and beyond

Explore how Nanolive’s LIVE T Cell Assay is accelerating immuno-oncology breakthroughs by enabling real-time visualization and quantification of T cell activity and target cell responses, in a single click.

In our latest webinar, From Live Cells to Insights: Nanolive’s Next-Gen Immune Profiling Solution, we showcased how this innovative assay is being applied across multiple immunotherapy applications, including:

• CAR-T efficacy

Narrow down the most potent CAR-T candidates for downstream in vivo testing through side-by-side performance comparison.

• CAR-T specificity

Evaluate clone selectivity across different antigens; observe Activation-Induced Cell Death (AICD) to gain deeper functional insights.

• Bispecific antibody function

Compare bispecific antibody candidates using real-time, quantitative metrics to assess functional performance.

Case study: Multiparametric insights from a single experiment. Label-free images were processed using the LIVE T Cell Assay. The images display digital overlays representing the activity and viability of the tested CAR-T cell clones. Multiparametric data can be extracted, including the percentage of active T cells, the lethal fraction of target cells, and more.

Don’t miss out! Explore these applications in detail by accessing the webinar on demand here!

Monitoring CAR-NK & T cell synergy with Nanolive

In a recent Journal for ImmunoTherapy of Cancer (JITC) publication, researchers used Nanolive’s 3D Explorer to visualize the real-time dynamics of 15×21 CAR-NK and T cells co-cultured with Raji lymphoma cells. The label-free imaging revealed that combining both cell types led to enhanced tumor killing compared to either alone. Nanolive’s technology enabled non-invasive monitoring of immune cell behavior, offering functional evidence of synergistic anti-tumor activity in a physiologically relevant environment.

To access the full paper: https://doi.org/10.1136/jitc-2024-010822

Latest publication highlights with Nanolive imaging:

• Immuno-oncology: Wang, X., Luo, W., Chen, Z., Li, C., Zhou, J., Huang, Z., Tang, L., Wu, J., Wu, Z., Li, Y., Zhang, Y., Kang, Y., Liu, Q., Xu, J., Xiong, W., Deng, J., Mei, H., & Hu, Y. (2024). Co-expression of IL-15 and CCL21 strengthens CAR-NK cells to eliminate tumors in concert with T cells and equips them with PI3K/AKT/mTOR signal signature. Journal for ImmunoTherapy of Cancer, https://doi.org/10.1136/jitc-2024-010822

• Microbiology: Pietrowska, A., Guźniczak, M., Kublicka, A., Matczuk, A., Wieliczko, A., Ulatowska-Jarża, A., & Buzalewicz, I. (2025). Efflux pump inhibitors from plant compounds: A new strategy to modulate bacterial growth and morphology, Journal of Industrial Crops and Products, https://doi.org/10.1016/j.indcrop.2025.121378

• Oncology: Chen, X.-Y., Chen, X., Liang, X.-H., Lu, D., Pan, R.-R., Xiong, Q.-Y., Liu, X.-X., Lin, J.-Y., Zhang, L.-J., Chen, H.-Z., Jin, J.-M., Zhang, W.-D., & Luan, X. (2025). Yuanhuacine suppresses head and neck cancer growth by promoting ASCT2 degradation and inhibiting glutamine uptake. Acta Pharmacologica Sinica, https://doi.org/10.1038/s41401-025-01562-2

• Cellular metabolism: Di Vincenzo, M., Campanati, A., Cannelonga, G., Orciani, M., & Diotallevi, F. (2025). Effects of Adalimumab on mitochondria of psoriatic mesenchymal stem cells. Journal of Cellular and Molecular Medicine, https://doi.org/10.1111/jcmm.70642

• Cardiac metabolism: Young, H.-Y., Lee, S., Choi, Y.-E., Nam, S.-H., Cha, S.-K., Jeong, Y., Kim, H., Shin, T.-M., & Park, K.-S. (2024). Hyperbaric oxygen increases mitochondrial biogenesis and function with oxidative stress in HL-1 cardiomyocytes. Journal of Applied Physiology, https://doi.org/10.1152/japplphysiol.00428.2024

Find over 300 publications featuring Nanolive imaging here.

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