Introducing the LIVE Cytotoxicity Assay: A breakthrough in investigative toxicology
Discover the forefront of drug safety and toxicology evaluation with Nanolive’s LIVE Cytotoxicity Assay Application Note. Our groundbreaking approach offers a label-free, high-content imaging solution that transforms the way cellular health, death, and phenotypic responses are monitored and quantified. Unlike traditional cytotoxicity assays, Nanolive’s technology bypasses the limitations of end-point measurements and fluorescent reporters, providing real-time, kinetic insights without the need for harmful dyes. With unparalleled precision and sub-cellular resolution, our assay facilitates a deeper understanding of drug interactions, ensuring more predictive and translational research outcomes.
Image: Cell death pathways as detected by the LCA
Cell death pathways as detected by the LCA. Adapted from Kim et al., Cell Death & Differentiation, 2019
Case Studies from the Investigative Toxicology Application Note
Discover the practical applications of Nanolive’s LIVE Cytotoxicity Assay through our detailed case studies. These examples illustrate the assay’s precision in analyzing cell health, death, and response to drugs, without the need for harmful dyes or labels. Learn how this approach provides clearer insights into cytotoxic effects, drug efficacy, and the mechanisms behind cell death, enhancing the accuracy of toxicological assessments and drug development processes.
Case study 1: Drug screening in a 96-well plate
Utilizes HeLa cells to evaluate the cytotoxic effects of varying concentrations of camptothecin and chloroquine, demonstrating the assay’s compatibility with high-throughput formats and its ability to discern concentration-dependent cell death kinetics.
Case study 2: Detecting cell death mechanisms
Focuses on identifying apoptosis, primary necrosis, and secondary necrosis in 3T3 pre-adipocytes, showcasing the assay’s unique capability to monitor different cell death pathways simultaneously, offering insights into the inflammatory profiles of drugs.
The LIVE Cytotoxicity Assay is a crucial tool for us in evaluating cell toxicity, especially as we focus on targeted cell apoptosis for our future products. At DSM for Personal Care, our key focus is pioneering innovative anti-aging technologies, with senolytics being the next generation of anti-aging ingredients. These ingredients selectively induce apoptosis in senescent cells, often referred to as zombie cells, which play a significant role in aging. The LIVE Cytotoxicity Assay seamlessly aligns with this objective, allowing us to dynamically track the reduction of these specific cells compared to the ones we want to preserve. This assay not only helps visualize the senolytic effects but also effectively differentiates between target and normal cells.
Key discoveries from Nanolive’s Investigative Toxicology Application Note
- Nanolive’s LIVE Cytotoxicity Assay’s unbiased, label-free quantification of cell health and death enhances drug safety evaluation.
- High-content, high-resolution imaging captures dynamic cellular responses, offering insights beyond traditional assays.
- Machine learning translates complex visual data into actionable, easy-to-understand metrics.
- Nanolive’s LIVE Cytotoxicity Assay enables the detection and quantification of various cell death mechanisms, including apoptosis and necrosis, in a single assay.
- Nanolive’s LIVE Cytotoxicity Assay supports screening in 96-wells, accelerating drug discovery and development processes.
Embark on a journey to redefine drug safety and investigative toxicology with Nanolive’s LIVE Cytotoxicity Assay. Our Investigative Toxicology Application Note opens new doors to understanding cellular behavior under drug influence, paving the way for more effective and safer therapeutic solutions. Dive into the detailed case studies and explore the key discoveries that position Nanolive’s technology at the cutting edge of toxicological research. Access the full application note now and learn how to set new standards for the future of drug evaluation and safety.
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