EVE Explorer quantifies cellular and organelle responses from label-free live cell images, with an intuitive automated workflow, customizable plotting options, and seamless data management, enabling researchers to make data-driven decisions and focus on discovery.

Efficient data organization and retrieval

AI-powered label-free cell segmentation

Label-free cell segmentation across different cell lines. Nanolive’s refractive index holotomographic images of human cervical cancer cells (HeLa), human induced pluripotent stem cells (iPSCs), human hepatocellular carcinoma cell line (HepG2) cells and preadipocytes (top), with digital cell segmentation overlays (bottom) following analysis performed with EVE Analytics.

EVE Explorer offers various AI-powered, application-specific digital assays that automatically segment cells and quantify morphological, and population features from Nanolive’s label-free holotomographic images. Trained across diverse cell lines, it eliminates manual processing, ensuring robust, reproducible, and high-throughput analysis for drug discovery, toxicity assessment, and cell therapy research, delivering reliable insights with zero user input.

Application-specific digital assay modules: Gather data on each cell in every frame, label-free!

Get higher significance earlier and faster with our panel of application-specific, push-button digital assays. Retroactive analysis is possible at any time.

EVE Analytics provides general metrics such as the growth rate, confluency, and compactness of cells over time enabling researchers to monitor drug-induced effects on cell cultures with high precision.

The LIVE Cytotoxicity Assay monitors cell health and distinguishes between living, apoptotic, and necrotic cells, facilitating drug screening and toxicity assessment.

The LIVE T Cell Assay monitors effector and target cell interactions and killing in co-culture, providing quantitative insights for immuno-oncology and cell therapy development.

The Smart Mitochondrial Assay^LIVE measures mitochondrial morphology, content, population and distribution, helping researchers assess mitochondrial function, metabolic activity, and drug-induced toxicity.

The Smart Lipid Droplet Assay^LIVE measures lipid droplet morphology, content, population and distribution, supporting metabolic research, obesity studies, and drug screening for lipid-related disorders.

Correlate molecular and structural changes for deeper analysis

In parallel with Nanolive’s standard label-free imaging, optional fluorescence imaging, visualization, and quantification allows users to extract an additional layer of insights from every single well. Automated analysis of fluorescent signals in EVE Explorer Pro allows you to:

• Visualize specific molecular targets
• Perform correlative imaging with refractive index images
• Define additional subpopulations within a single well by fluorescence signal
• Quantify change in cellular fluorescence signal over time e.g. gene expression

NanoPlot – Unleash the full potential of Nanolive high-content imaging data

Features overview

Choose the version of EVE Explorer that best matches your research goals:

Andrew Martins, Ph.D.​
Assistant Professor, Immunobiology ​
Director of Technology, Center for Systems and Engineering Immunology​
Yale School of Medicine

“With further improved object segmentation capability, integrated analysis features that reduce our need to use external software, and new project management capabilities, EVE Explorer is allowing us to more quickly gain insight into our live cell experiments.”