Smart Mitochondrial AssayLIVE
Redefining Mitochondrial Research: Non-Invasive Imaging & 1-Click Analysis
Powered by high-resolution label-free imaging and AI-driven analysis, the Smart Mitochondrial AssayLIVE is the world’s first fully automated solution for noninvasive, quantitative assessment of mitochondrial dynamics. Created to accelerate drug discovery, it provides deeper insights into mitochondrial responses without any need for dyes or complex sample preparation.
One-click assay
Time-lapse imaging without phototoxicity
Capture dynamic mitochondrial responses
Quantify morphological
changes at mitochondrion and network levels
Correlative fluorescence and functional analyses
Unique, comprehensive metrics
Mitochondria in Nanolive’s holotomographic images are automatically segmented and measured to produce more than 30 label-free metrics per mitochondrion and per cell, including mitochondrial:
These comprehensive, dye-free insights empower biopharma researchers to assess critical mitochondrial dynamics, such as fission, fusion, swelling, mitophagy, and network complexity, key for evaluating metabolic function, cellular stress, and drug-induced toxicity with unmatched precision.
Refractive Index
Mitochondrial segmentation
Integrated Analysis
Label-free mitochondrial segmentation in HeLa cells. Nanolive’s refractive index holotomographic images of HeLa cells at the population level, with a zoomed-in view of a single cell. Refractive index image alone (left) and with digital mitochondrial overlays (center) following analysis performed with the Smart Mitochondrial AssayLIVE. On the right we display an analysis output showing the relationship between mitochondrial dry mass, count, and time. Both metrics decrease in tandem, confirming that mitochondria within the cells are being progressively degraded.
Multiplex with integrated fluorescence
In parallel with Nanolive’s standard label-free imaging, optional fluorescence imaging, visualization, and quantification provide researchers with an additional layer of actionable insights from every well. Automated analysis in EVE Explorer Pro enables:
- Correlative fluorescence imaging with refractive index-based structures, to associate protein expression with morphological properties for deeper insights.
- Definition of distinct cell sub-populations in co-cultures by fluorescence signal, enabling comparison of responses across specific cell types.
- Quantification of fluorescence changes over time allowing for the monitoring of dynamic functional responses, such as ROS production, to evaluate drug efficacy and toxicity.
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Applications
Evaluate the efficacy of mitochondrial
targeting therapies
Monitor uptake of fluorescent drugs,
nanoparticles, or therapeutic mitochondria
Use mitochondrial response as a marker
for investigative toxicology
Characterize mitochondrial dysfunction by
studying fragmentation, swelling and
network disruption
Product Presentation
Discover the World’s First 1-Click, Label-Free Mitochondrial Assay
Mitochondria are key indicators of cellular responses but are easily perturbed by traditional markers, leading to potential biases and imaging limitations. Nanolive’s Smart Mitochondrial AssayLIVE overcomes these challenges with label-free, non-invasive, high-content imaging—first demonstrated in Nature Communications (2024) and now available as a single-click digital assay.
Watch our online launch event on demand to see how this AI-powered analysis detects mitochondrial morphology changes at both individual and network levels in response to drug treatments and phototoxicity. Learn how our new EVE Explorer analysis environment enables automated workflows, customizable data visualization, and seamless data management for live-cell, high-content analysis.
The latest research from Nanolive’s R&D department and the Institut Pasteur, recently published in Nature Communications, uses cutting-edge AI-based computer vision to give new insights into organelle network disruption during coronavirus infection.
Building on the expertise used to develop Nanolive’s existing digital assays for cellular image analysis, members of Nanolive’s team created new automated methods for detecting and quantifying cell organelles in label-free holotomographic images. These digital segmentations were then used to quantify organelle numbers, their changing morphology, interactions, mass, and other features.
Nanolive’s Imaging Technology
Nanolive delivers breakthrough label-free live cell imaging and analysis solutions that accelerate research in growth industries such as drug discovery and cell therapy. Nanolive’s innovative solutions combine screening, imaging and analysis to radically advance how scientists study living cells and provide novel biological insights such as the mechanisms of cancer and metabolic diseases.
Our imaging platforms use holotomography to capture label-free timelapse images of cells and their organelles to give insight into dynamic processes and phenotypic changes. But what is holotomography, how does it work and what benefits does this live-cell imaging approach bring to fundamental research and drug development?
Download our free eBook, which dives into the technology behind Nanolive’s high-content live cell images and explains how holotomography is essential for providing deep phenotypic insights into cellular responses.
Stefan Milde
Imaging Lead
Bit.bio
“The introduction of the label-free Smart Mitochondrial AssayLIVE is a very exciting development, delivering a range of metrics that allow us to interrogate the dynamics of mitochondrial biology in undisturbed, live cells in unprecedented detail. This new module will be an invaluable tool for the deep phenotyping of our opti-ox powered human iPSC-derived cells and disease models.”
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