SMART MITOCHONDRIAL ASSAY LIVE
Detect mitochondrial stress
before viability changes
Label-free, automated quantification of mitochondrial
dynamics in living cells
See mitochondrial stress hours before cell viability drops
Understand mechanisms of action with dynamic mitochondrial profiling
Preserve native biology with label-free, non-phototoxic imaging
Standardize results with AI-driven, automated analysis
Generate decision-ready data faster and with greater confidence
“…[the software] significantly accelerated our data analysis workflow … [It] enabled us to rapidly obtain objective and reproducible quantitative metrics”
Dai-Wei Hu
China Medical University
Automated analysis & key readouts
From image acquisition to biological insight, EVE Explorer and the Smart Mitochondrial AssayLive transform complex image data into decision-ready results with:
- AI-powered segmentation and classification
- 30+ quantitative mitochondrial and cellular metrics
- Dynamic analysis over time
- Population, single cell, and mitochondrion-level insights
1. Label-free imaging
2. AI segmentation
3. Automated analysis
✓ Detect stress earlier
✓ Understand MoA
✓ Compare compounds
✓ Make confident decisions
Key mitochondrial readouts
Fission &
Fusion
Swelling &
Fragmentation
Network
complexity
Mitophagy &
Biogenesis
Mitochondrial mass
& Content
Case studies
Monitoring early
cellular stress
Mitochondrial network fragmentation, fission and mitophagy provide early warning signs of cellular stress when compared to morphological markers of cell death at the whole-cell level.
Mitochondrial length as a biomarker in breast cancer
The Smart Mitochondrial Assay LIVE was used alongside molecular biology methods to show an association of the chemical DEHP with early onset breast cancer.
Differentiating virus variants by organelle network dynamics
Organelles including the nucleus, lipid droplets and mitochondria have distinct phenotypic responses to infection by different coronavirus variants.
Want to discuss your specific use-case? Ask one of our scientists here
Trusted globally & featured in more than 450 peer-reviewed publications
Peer-reviewed
publications
Smart Mitochondrial Assay References
China Medical University
SLC6A14 Drives Mitochondrial Fusion and Oxidative Phosphorylation to Promote Cancer Stemness and Early-Onset of Breast Cancer Advanced Science (2026)
Institut Pasteur & Nanolive
Dynamic label-free analysis of SARS-CoV-2 infection reveals virus-induced subcellular remodeling
Nature Communications (2024)
Platform compatibility
The Smart Mitochondrial AssayLIVE is available on Nanolive’s 3D Cell Explorer platform for high content, high-resolution live cell imaging.
3D Cell Explorer 96focus
Move Forward with Confidence
Whether you’re optimizing early discovery assays or investigating complex cellular responses, Nanolive helps you see more, earlier – without compromising biology.