Powered by high-resolution label-free imaging and AI-driven analysis, the Smart Mitochondrial Assay^LIVE is the world’s first fully automated solution for noninvasive, quantitative assessment of mitochondrial dynamics. Created to accelerate drug discovery, it provides deeper insights into mitochondrial responses without any need for dyes or complex sample preparation.

Correlative fluorescence and functional analyses

Unique, comprehensive metrics 

Mitochondria in Nanolive’s holotomographic images are automatically segmented and measured to produce more than 30 label-free metrics per mitochondrion and per cell, including mitochondrial:

• number of endpoints

• number of junctions

These comprehensive, dye-free insights empower biopharma researchers to assess critical mitochondrial dynamics, such as fission, fusion, swelling, mitophagy, and network complexity, key for evaluating metabolic function, cellular stress, and drug-induced toxicity with unmatched precision.

Integrated Analysis

Label-free mitochondrial segmentation in HeLa cells. Nanolive’s refractive index holotomographic images of HeLa cells at the population level, with a zoomed-in view of a single cell. Refractive index image alone (left) and with digital mitochondrial overlays (center) following analysis performed with the Smart Mitochondrial Assay^LIVE. On the right we display an analysis output showing the relationship between mitochondrial dry mass, count, and time. Both metrics decrease in tandem, confirming that mitochondria within the cells are being progressively degraded.

Multiplex with integrated fluorescence

In parallel with Nanolive’s standard label-free imaging, optional fluorescence imaging, visualization, and quantification provide researchers with an additional layer of actionable insights from every well. Automated analysis in EVE Explorer Pro enables:

• Correlative fluorescence imaging with refractive index-based structures, to associate protein expression with morphological properties for deeper insights.
• Definition of distinct cell sub-populations in co-cultures by fluorescence signal, enabling comparison of responses across specific cell types.
• Quantification of fluorescence changes over time allowing for the monitoring of dynamic functional responses, such as ROS production, to evaluate drug efficacy and toxicity.

Applications