Scientific Publications
Peer-reviewed research using Nanolive imagingDiscover a list of peer-reviewed scientific publications using Nanolive imaging in the fields of drug discovery, cell metabolism, etc.
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Feature Application: Characterization of single cells at the population level
In this Feature Application, we showcase the enormous potential that Nanolive live cell imaging holds for single cell characterization. We begin, by analyzing micro-heterogeneity at the population and the temporal level in unperturbed cells (case study one). We then extend our analysis to include a quantitative assessment of lipid droplet dynamics (case study two), before investigating how intracellular trafficking (case study three) and respiratory perturbation (case study four) impacts microheterogeneity in cell morphology.
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Feature Application: Characterization of neurons
This Feature Application shows the huge potential that Nanolive cell imaging holds for neurobiological research. Our first case study shows a timeline of the morphological changes undifferentiated primary cortical neurons undergo after exposure to neurite stimulation media. High precision segmentations are used to calculate cell metrics (e.g. volume, shape and dry mass) of interest. These calculations are directly linked to novel behaviours observed in individual neurons. Our second and third case studies focus on the sub-cellular morphological changes that stem cells undergo during neural differentiation, at both the single cell (case study 2) and population level (case study 3).
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Feature Application: Live T Cell Assay
The Live T Cell Assay promises to accelerate the advancement of basic cell biology and of novel, safe, and effective immunotherapies. The goal of this application note is to show some of the potential uses of the Live T Cell Assay and hopefully, inspire research that will increase the success rate of anticancer drug development. To this end, we present the first in-depth investigation of bispecific antibodies using the Live T Cell Assay.
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Feature Application: Multiparametric cytotoxicity assays
This Feature Application shows the huge potential that Nanolive imaging holds for the drug discovery process, where cytotoxicity remains one of the major causes of drug withdrawal, and there is an urgent need for reliable and time-saving assay workflows. Our first case study features an in-depth investigation of the dynamics of cell death following exposure to seven cell death stimuli: extreme shifts in external pH, thermal stress, phototoxicity, oxidative stress, Shikonin, Ebastine, and Taxol. Our second case study shows that we can quantify drug-dependent and dose-dependent effects in 96-well plate format.
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Occurrence of lymphangiogenesis in peripheral nerve autografts contrasts Schwann cell-induced apoptosis of lymphatic endothelial cells in vitro
Authors: Carina Hromada, Jaana Hartmann, Johannes Oesterreicher, Anton Stoiber, Anna Daerr, Barbara Schädl, Eleni Priglinger, Andreas H. Teuschl-Woller, Wolfgang Holnthoner, Johannes Heinzel, David Hercher
https://doi.org/10.3390/biom12060820, 2022
Published in: Biomolecules
Tags: peripheral nerve regeneration, lymphangiogenesis, Schwann cells, lymphatic endothelial cells
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Modulation of mitochondrial electron transport chain by pyroptosis nanoagonists for photoresponsive tumor destruction
Authors: Penghao Ji, Shenyan Zhang, Peilei Liu, Xi Li, Weichao Bao, Xiangzhi Cui, Minfeng Huo, Jianlin Shi
https://doi.org/10.1016/j.nantod.2022.101511, 2022
Published in: Nanotoday
Tags: Pyroptosis; Oligomycin A; Mitochondrial damage; Oxidative stress; Mild hyperthermia
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Legumain/pH dual-responsive lytic peptide–paclitaxel conjugate for synergistic cancer therapy
Authors: Shanshan Zheng, Yue Cai, Yulu Hong, Yubei Gong, Licheng Gao, Qingyong Li, Le Li, Xuanrong Sun
https://doi.org/10.1080/10717544.2022.2081380, 2022
Published in: Drug Delivery
Tags: Peptide–drug conjugate; legumain-responsive; pH-responsive; synergistic cancer therapy
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Interleukin-13 and its receptor are synaptic proteins involved in plasticity and neuroprotection
Authors: Shun Li, Florian olde Heuvel, Rida Rehman, Zhenghui Li, Oumayma Aousji, Albrecht Froehlich, Wanhong Zhang, Alison Conquest, Sarah Woelfle, Michael Schoen, Caitlin O´Meara, Richard Lee Reinhardt, David Voehringer, Jan Kassubek, Albert Ludolph, Markus Huber-Lang, Bernd Knöll, Maria Cristina Morganti-Kossmann, Tobias Boeckers, Francesco Roselli
https://doi.org/10.1101/2021.12.01.470746, 2021
Published in: bioRxiv
Tags: IL-13, synapses, CREB, excitotoxicity, traumatic brain injury
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ATP purinergic receptor P2X1-dependent suicidal NETosis induced by Cryptosporidium parvum under physioxia conditions
Authors: Seyed Sajjad Hasheminasab; Iván Conejeros; Zahady D. Velásquez; Tilman Borggrefe; Ulrich Gärtner; Faustin Kamena; Anja Taubert; Carlos Hermosilla
https://doi.org/10.3390/biology11030442, 2022
Published in:Biology
Tags: Cryptosporidium parvum; NETosis; purinergic receptor P2X1; Notch; glycolysis; PMN
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N-Acetyl-Cysteine Increases Activity of Peanut-Shaped Gold Nanoparticles Against Biofilms Formed by Clinical Strains of Pseudomonas aeruginosa Isolated from Sputum of Cystic Fibrosis Patients
Authors: Ewelina Piktel; Urszula Wnorowska; Joanna Depciuch; Dawid Łysik; Mateusz Cieśluk; Krzysztof Fiedoruk; Joanna Mystkowska; Magdalena Parlińska-Wojtan; Paul A Janmey; Robert Bucki
https://doi.org/10.2147/IDR.S348357, 2022
Published in: Infection and Drug Resistance
Tags: gold nanoparticles, non-spherical nanoparticles, N-acetyl-cysteine, bacteria biofilm, cystic fibrosis, Pseudomonas aeruginosa