Scientific Publications

Authors: Maria do Carmo Greier; Annette Runge; Jozsef Dudas; Viktoria Pider; Ira-Ida Skvortsova; Dragana Savic; Herbert Riechelmann

https://doi.org/10.1038/s41598-022-16829-5, 2022

Published in: Scientific Reports

Tags: mitochondria; mesenchymal stem cells; head neck cancer

Authors: Jiahui Zhao; Kaiyue Liang; Hao Zhong; Shizhu Liu; Peilong Sun; Rongjun He

https://doi.org/10.1016/j.ijbiomac.2022.07.098, 2022

Published in: International Journal of Biological Macromolecules

Tags: G. frondosa; Polysaccharide-protein complex; Cold-water extraction; Structural analysis; HepG2 cell inhibitory activity

Authors: Sheng-Chieh Hsu; Nian-Ping Wu; Yi-Ching Lu; Yunn-Hwa Ma

https://doi.org/10.3390/pharmaceutics14081523, 2022

Published in: Pharmaceutics

Tags: magnetic nanoparticles; endocytosis; epigallocatechin gallate; 67LR; Akt

Authors: A.A. Zhikhoreva, A.V. Belashov, T.N. Belyaeva, A.V. Salova, I.K. Litvinov, E.S. Kornilova, I.V. Semenova, O.S. Vasyutinskii

https://doi.org/10.1016/j.pdpdt.2022.102973, 2022

Published in: Photodiagnosis and Photodynamic Therapy

Tags: photodynamic treatment, malignant cells, fluorescent analysis, digital holographic microscopy, phase images

Authors: Johanna M. Buschhaus, Shrila Rajendran, Brock A. Humphries, Alyssa C. Cutter, Ayşe J. Muñiz, Nicholas G. Ciavattone, Alexander M. Buschhaus, Tatiana Cañeque, Zeribe C. Nwosu, Debashis Sahoo, Avinash S. Bevoor, Yatrik M. Shah, Costas A. Lyssiotis, Pradipta Ghosh, Max S. Wicha, Raphaël Rodriguez, Gary D. Luker

https://doi.org/10.1038/s41388-022-02385-9, 2022

Published in: Oncogene

Tags: breast cancer, stem cells, iron, lysosome, drug resistance, estrogen-targeted therapy

Authors: Yuqi Wang, Lingpu Zhang, Yan Liu, Linlin Tang, Juan He, Xiaqing Sun, Muhsin H. Younis, Daxiang Cui, Haihua Xiao, Dong Gao, Xiang-Yang Kong, Weibo Cai, Jie Song

https://doi.org/10.1002/adhm.202201178, 2022

Published in: Advanced Healthcare Materials

Tags: macrophages; antitumor; drug delivery; immunology; immunotherapy; nanomedicine

In this application note, we showcase some of the potential uses of the Smart Lipid Droplet Assay (SLDA) including quantifying signs of aging in human skin cells (case study 1); investigating LD dynamics during foam macrophage formation (case study 2); examining LD dry mass dynamics in cancer cells (case study 3), and dissecting the details of LD biogenesis (case study 4).

In this Technical Note, we introduce the Smart Lipid Droplet Assay (SLDA) and showcase its output in multiple cell types at varying confluences and across different field of view sizes. We then compare the performance of the SLDA against the fluorescence marker LipidSpot 610, before finishing with a case study where we use the SLDA to quantify how oleic acid (OA) addition changes LD dynamics in pre-adipocyte cells.

In this document, we demonstrate how the CX-A can be used in drug screening. We go through the whole process from image acquisition to data analysis and interpretation. As a case study, we chose to test what extent the art of observing influences the outcome of our experiments; something too often ignored when running live cell experiments. We measured the effects on cell health of four different cell treatments (1) label-free imaging and vehicle; (2) label-free imaging and addition of Mitotracker; (3) fluorescence imaging at low intensity regime and addition of Mitotracker; (4) fluorescence imaging at high intensity regime and addition of Mitotracker, using EA.

This technical note explores these challenges, and presents Nanolive’s cutting edge, AI-assisted solution, the Live T Cell Assay. It also includes a full use case study where the Live T Cell Assay is used to quantify the effect a bispecific antibody has on T cell and cancer cell dynamics.