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CX-A

3D Cell Explorer 96focus

 

CX-A

CX-A

 

3D Cell Explorer-fluo

3D Cell Explorer-fluo

 

3D Cell Explorer

3D Cell Explorer

All assays

EVE Analytics & Digital Assays

 

Support for your  3D Cell Explorer 96focus

CX-A
Pre-requisites

Pre-installation guide and checklist. Download here.

Installation

The installation of your 3D Cell Explorer 96focus needs to be performed by a trained technician. Please contact Nanolive Support or your local distributor to schedule the installation of your device.

Make sure that the prerequisites are ready for the date of installation.

Accessories
  • Your  3D Cell Explorer 96focus is delivered with a TokaiHit incubator. Click here to download the related documentation.
  • Your 3D Cell Explorer 96focus is designed to work with Nanolive’s custom 96-well plates and won’t work with any other plates you can find on the market. Please contact Nanolive Sales (sales@nanolive.ch) to order.
  • Find out more information about the different configurations of the fluorescence filter and fluorophores. Click here.
Manual

3D Cell Explorer 96focus User Guide Download here

3D Cell Explorer 96focus User Manual. Download here.

For more information, you can check our application note page here.

Maintenance and Troubleshooting

Download the cleaning procedure for the optical elements here.

For support on your 3D Cell Explorer 96focus, please contact Nanolive support.

Service Contracts

To ensure that your device will keep working as well as on day one, we offer a service contract ensuring peace of mind and total control over maintenance costs.
We offer two different Tiers depending on your needs and region:

For more information, please download our flyer here.

Downloads

Pre-installation guide and checklist. Download here.

Tokaihit Top Stage incubator manual. Download here.

Fluorescence filter and fluorophores configuration. Click here.

Cleaning procedure for the optical elements. Download here.

Support for your CX-A

CX-A
Pre-requisites

Pre-installation guide and checklist. Download here.

Installation

The installation of your CX-A needs to be performed by a trained technician. Please contact Nanolive Support or your local distributor to schedule the installation of your device.

Make sure that the prerequisites are ready for the date of installation.

Accessories
  • Your CX-A is delivered with a TokaiHit incubator. Click here to download the related documentation.
  • Your CX-A is designed to work with Nanolive’s custom 96-well plates and won’t work with any other plates you can find on the market. Please contact Nanolive Sales (sales@nanolive.ch) to order.
  • Find out more information about the different configurations of the fluorescence filter and fluorophores. Click here.
Software download

To download and install the latest version of EVE software to use with your CX-A and EVE Analytics, click the following link.

If you need assistance, please please contact Nanolive Support or your local distributor.

Release notes for EVE: click here.

How to run an experiment

This section includes the necessary videos and documentation that you might need during different steps while running an experiment with the CX-A: from the sample preparation to the data analysis.

EVE Tutorial – Part 1 – Start an acquisition

EVE Tutorial – Part 2 – How to run an Acquisition

EVE Tutorial – Part 3 – How to navigate through an acquisition

EVE Tutorial – Part 4 – How to export an acquisition

Short sample preparation guide for the CX-A. Download here.

Manual and user guide

CX-A user’s manual. Download here.

CX-A user guide. Download here.

For more information, you can check our application note page here.

Maintenance and Troubleshooting

Download the cleaning procedure for the optical elements here.

For support on your CX-A, please contact Nanolive support.

Service Contracts

To ensure that your device will keep working as well as on day one, we offer a service contract ensuring peace of mind and total control over maintenance costs.
We offer two different Tiers depending on your needs and region:

For more information, please download our flyer here.

Downloads

Pre-installation guide and checklist. Download here.

Tokaihit Top Stage incubator manual. Download here.

Fluorescence filter and fluorophores configuration. Click here.

Short sample preparation guide for the CX-A. Download here.

CX-A user guide. Download here.

Cleaning procedure for the optical elements. Download here.

Support for your 3D Cell Explorer-fluo (CX-f)

3D Cell Explorer-fluo
Pre-requisites

Computer requirements. Download here.

Installation

The installation of your 3D Cell Explorer is fast and easy. Follow the steps in the video tutorial below.

Set up your 3D Cell Explorer-fluo

Set up your Nanolive Top Stage Incubator

Installation of the Nanolive Objective Heater on your 3D Cell Explorer-fluo

3D Cell Explorer-fluo user manual. Download here.

3D Cell Explorer-fluo user guide. Download here.

Incubator Quick installation and user guide. Download here.

Top Stage Incubator manual. Download here.

H301- MINI chamber manual. Download here.

 

Accessories

Your 3D Cell Explorer-fluo is delivered with the HiGrade stage. Follow the instructions below for its installation.

Download the HiGrade stage mounting procedure here.

Software download

In order to run your 3D Cell Explorer-fluo you need to download and install the latest STEVE software on your computer. Make sure the computer you are planning to use meets the specifications you can find here. Note that a functioning internet connection will be required during all the installation and setup process.

Download the STEVE software here.

Release notes Software: click here.

How to run an experiment

This section includes the necessary documentation that you might need during different steps while running an experiment with the 3D Cell Explorer-fluo: from the sample preparation to the data analysis.

Sample preparation video:

Download the Labware compatibility report here.

Download the sample preparation manual here.

Tips and tricks

Your first acquisition with the 3D Cell Explorer:

How to focus on your cells:

For more information, visit our application note page here.

Maintenance and Troubleshooting

Download the cleaning procedure for the optical elements here.

6 steps to troubleshoot an issue

BEFORE following the next 6 troubleshooting steps, please make sure that:

Step 1: Your computer is connected to the 3D Cell Explorer and internet

Step 2: You verify that you have the configuration file in this Location C:Users”your user name”AppDataRoamingNanoliveconfig

1) Check if the 3D Cell Explorer’s driver on Windows 10 are up to date
  1. Install Steve, connect all cables and switch on the microscope.
  2. Open the Device Manager (try one of the 7 ways to do it from here).
  3. Open the “Ports (COM & LPT)”. You should get something similar to this figure:
    NL_TroubleShoot1
  4. Right click on “USB Serial Device (COM_XXX)” and choose “Update Driver Software…” .
  5. Now, choose “Search automatically for updated driver software” and wait until windows updates to “3D Cell Explorer (COM_XXX)”. At the end you should see this:
    NL_TroubleShoot1
  1. You are done. You can launch Steve now.
2) Check if the 3D Cell Explorer is installed
  1. Open the Device Manager: Click on the Windows Start Button, write “devmgmt.msc” and push enter or follow the instruction from: http://pcsupport.about.com/od/tipstricks/f/finddevmngr.htm. On the Device Manager under the Ports (COM & LPT) you should find the 3D Cell Explorer (ignore the COM port number).
    NL_TroubleShoot2
3) Check if the Basler Camera is installed
  1. On the Device Manager you should find:
    NL_TroubleShoot3
4) Check if the USB 3.0 Drivers are installed

On the Device Manager under the Universal Serial Bus controllers you should find something similar with this image (Intel(R) could be replaced by other manufactures, like Renesas Electronics, for example):
NL_TroubleShoot4

5) Check if the camera USB 3.0 cable is connected to an USB 3.0 port

Please check if one of your computer USB 3.0 ports presents either the USB 3.0 symbol (Image B) or the blue plastic inside (Image C). If yes, please connect the camera USB cable to this port. If no, your computer is not equipped with USB 3.0 ports.
NL_TroubleShoot5

6) Check your NVIDIA driver version

From your system tray (right side of the windows taskbar) click on the “NVIDIA GeForce Experience”. If you do not find it, then the Nvidia card drivers are not installed.
If you found it: click on “Open NVIDIA Control Panel”. On the NVIDIA Control Panel click on the left bottom link: System Information. From there, please report the following Graphics card information:

  • your graphic card (under the left side Items list),
  • the “Driver version” (under the right side Details list)
  • the “Dedicated video memory” (under the right side Details list, scroll down to find it).

NL_TroubleShoot6

 

 

Service Contracts

To ensure that your device will keep working as well as on day one, we offer a service contract ensuring peace of mind and total control over maintenance costs.
We offer two different Tiers depending on your needs and region:

For more information, please download our flyer here.

Downloads

Computer requirements. Download here.

3D Cell Explorer-fluo user manual. Download here.

3D Cell Explorer-fluo user guide. Download here.

Incubator Quick installation and user guide. Download here.

Top Stage Incubator manual. Download here.

H301- MINI chamber manual. Download here.

Download the HiGrade stage mounting procedure here.

Download the STEVE software here.

Download the Labware compatibility report here.

Download the sample preparation manual here.

Download the cleaning procedure for the optical elements here.

Support for your 3D Cell Explorer (CX)

3D Cell Explorer
Pre-requisites

Computer requirements. Download here.

Installation

The installation of your 3D Cell Explorer is fast and easy. Follow the steps in the video tutorial below.

Set up your 3D Cell Explorer

User manual 3D Cell Explorer. Download here.

Accessories

Your 3D Cell Explorer can be delivered with the optional HiGrade stage. Follow the instructions below for its installation.

Download the HiGrade stage mounting procedure here.

Software download

In order to run your 3D Cell Explorer you need to download and install the latest STEVE software on your computer. Make sure the computer you are planning to use meets the specifications you can find here. Note that a functioning internet connection will be required during all the installation and setup process.

Download the STEVE software here.

How to run an experiment

This section includes the necessary documentation that you might need during different steps while running an experiment with the 3D Cell Explorer: from the sample preparation to the data analysis.

Sample preparation video

Download the Labware compatibility report here.

Download the sample preparation manual here.

Tips and tricks

Your first acquisition with the 3D Cell Explorer

How to focus on your cells

For more information, visit our application note page here.

Maintenance and Troubleshooting

Download the cleaning procedure for the optical elements here.

6 steps to troubleshoot an issue

BEFORE following the next 6 troubleshooting steps, please make sure that:

Step 1: Your computer is connected to the 3D Cell Explorer and internet

Step 2: You verify that you have the configuration file in this Location C:Users”your user name”AppDataRoamingNanoliveconfig

1) Check if the 3D Cell Explorer’s driver on Windows 10 are up to date
  1. Install Steve, connect all cables and switch on the microscope.
  2. Open the Device Manager (try one of the 7 ways to do it from here).
  3. Open the “Ports (COM & LPT)”. You should get something similar to this figure:
    NL_TroubleShoot1
  4. Right click on “USB Serial Device (COM_XXX)” and choose “Update Driver Software…” .
  5. Now, choose “Search automatically for updated driver software” and wait until windows updates to “3D Cell Explorer (COM_XXX)”. At the end you should see this:
    NL_TroubleShoot1
  1. You are done. You can launch Steve now.
2) Check if the 3D Cell Explorer is installed
  1. Open the Device Manager: Click on the Windows Start Button, write “devmgmt.msc” and push enter or follow the instruction from: http://pcsupport.about.com/od/tipstricks/f/finddevmngr.htm. On the Device Manager under the Ports (COM & LPT) you should find the 3D Cell Explorer (ignore the COM port number).
    NL_TroubleShoot2
3) Check if the Basler Camera is installed
  1. On the Device Manager you should find:
    NL_TroubleShoot3
4) Check if the USB 3.0 Drivers are installed

On the Device Manager under the Universal Serial Bus controllers you should find something similar with this image (Intel(R) could be replaced by other manufactures, like Renesas Electronics, for example):
NL_TroubleShoot4

5) Check if the camera USB 3.0 cable is connected to an USB 3.0 port

Please check if one of your computer USB 3.0 ports presents either the USB 3.0 symbol (Image B) or the blue plastic inside (Image C). If yes, please connect the camera USB cable to this port. If no, your computer is not equipped with USB 3.0 ports.
NL_TroubleShoot5

6) Check your NVIDIA driver version

From your system tray (right side of the windows taskbar) click on the “NVIDIA GeForce Experience”. If you do not find it, then the Nvidia card drivers are not installed.
If you found it: click on “Open NVIDIA Control Panel”. On the NVIDIA Control Panel click on the left bottom link: System Information. From there, please report the following Graphics card information:

  • your graphic card (under the left side Items list),
  • the “Driver version” (under the right side Details list)
  • the “Dedicated video memory” (under the right side Details list, scroll down to find it).

NL_TroubleShoot6

 

 

Service Contracts

To ensure that your device will keep working as well as on day one, we offer a service contract ensuring peace of mind and total control over maintenance costs.
We offer two different Tiers depending on your needs and region:

For more information, please download our flyer here.

Downloads

Computer requirements. Download here.

User manual 3D Cell Explorer. Download here.

Download the HiGrade stage mounting procedure here.

Download the STEVE software here.

Download the Labware compatibility report here.

Download the sample preparation manual here.

Download the cleaning procedure for the optical elements here.

 

Support for EVE Analytics and Digital Assays

All assays
EVE Analytics

EVE Analytics requires the installation of the EVE software. Click on the following link to download and install the latest EVE version compatible with EVE Analytics.

In order to request and install your Eve Analytics Application Module follow the procedure here.

EVE Analytics user guide. Download here.

Application modules release notes: click here.

Live T Cell Assay

Live T Cell Assay user guide. Download here.
Live T Cell Assay sample preparation guide. Download here.

Smart Lipid Droplet Assay

Smart Lipid Droplet Assay user guide. Download here.

LIVE Cell Death Assay

LIVE Cell Death Assay​ user guide. Download here.