Newsletter November 2024: Techniques for high-content live cell imaging

Welcome to the November edition of the AI for Live Cell Insights Newsletter, bringing you the latest live cell analyses powering drug discovery and cosmetics development. Each month, we will explore applications of AI-based cellular analysis for label-free live cell imaging, with publication highlights and news from Nanolive. This month, we are highlighting the application of label-free holotomography for mitochondrial analysis, as well as recent publications in the fields of cosmetics and metabolism.

A ground-breaking tool for label-free mitochondrial analysis

Our June Newsletter focused on a Nature Communications paper by scientists at Nanolive and Institut Pasteur which featured AI analysis of organelle network dynamics, including mitochondria for the first time. ‘Dynamic label-free analysis of SARS-CoV-2 infection reveals virus-induced subcellular remodeling’ presented new automated methods for detecting and quantifying cell nuclei, nucleoli and mitochondria in label-free holotomographic images. These digital segmentations of organelles were then used to quantify organelle numbers, their changing morphology, interactions, mass, and other features, and made it possible to distinguish between cellular responses to different coronavirus strains.

If you wondered when this mitochondrial analysis technology would be available, you are in luck! Nanolive have announced an upcoming tool for automated, label-free mitochondrial analysis, and you can receive early updates on the launch by signing up here.

New eBook: Techniques for high-content live cell imaging

High-content images contain a wealth of data, and automated image analysis can extract hundreds of metrics from a single frame, such as cell area, dry mass, and details of organelle networks. Capturing this information through imaging can be carried out in many ways, from label-free methods to cell painting, and high-content imaging is a fast-moving, innovative field.

Do you want to compare label-free and fluorescence microscopy methods to find the best fit for high-content analysis, but don’t know where to start? This eBook compares different live cell imaging methods to answer questions like “How do different imaging methods affect cell viability?” and “How can high-content images be analyzed?”

The following live cell imaging methods are compared:

• • Widefield fluorescence
  • Point scanning and spinning disk confocal
  • Light sheet microscopy (SPIM)
  • Phase contrast
  • Quantitative phase imaging (QPI)
  • Nanolive’s holotomography

Click to download the eBook: Techniques for high-content live cell imaging

Latest publication highlights with Nanolive imaging:

Lipotoxicity: Peng, C. C., et al. (2024) ‘Molecular mechanism of ectopic lipid accumulation induced by methylglyoxal via activation of the NRF2/PI3K/AKT pathway implicates renal lipotoxicity caused by diabetes mellitus’ Plos One DOI: 10.1371/journal.pone.0306575

Cosmetics: Martínez-Gutiérrez, A., et al. (2024) ‘New Chalcone-Derived Molecule for the Topical Regulation of Hyperpigmentation and Skin Aging’, Pharmaceutics DOI: 10.3390/pharmaceutics16111405

Extract from Figure 3. Anti-glycation and wound healing effects of compound 1. Martínez-Gutiérrez, A., et al. (2024). Used under Creative Commons license (CC BY).

Drug discovery: Gu, W.-J., et al. (2024) ‘TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy’ Theranostics DOI: 10.7150/thno.97662

Lipid droplets: Sherman, D., et al (2024) ‘PNPLA3-I148M is a Neomorph that Interferes with Two Primary Hepatic Triglyceride Clearance Pathways’ bioRxiv DOI: 10.1101/2024.10.29.620978

Cell characterization: Leineweber, W., et al, (2024) ‘Holotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization’ bioRxiv DOI: 10.1101/2024.11.04.621934

Oncology: Charalampopoulou, A. et al. (2024) ‘Vaginal mucosal melanoma cell activation in response to photon or carbon ion irradiation’, International Journal of Particle Therapy DOI: 10.1016/j.ijpt.2024.100630

Bacteria: Seungyeon Byun, A. et al. (2024) ‘Respiratory Delivery of Lacticaseibacillus rhamnosus GG by Vibrating-Mesh and Jet Nebulisation’, Pharmaceutics DOI: 10.3390/pharmaceutics16101326

Find over 250 publications featuring Nanolive imaging here.

Read our latest news