Tumor-derived extracellular vesicles uptake in mouse macrophages

Tumor-associated macrophages secrete extracellular vesicles (EVs) that range from a variety of sizes and subcellular origins[1]–[5].

Exosomes (30-150nm) are a type of EVs originated from endosomal multivesicular bodies[4], [6]. Other types of EVs result from the shedding of plasma membrane, which leads to the formation of microvesicles (100-1000nm) during steady-state conditions or to apoptotic bodies (100-5000nm) during cell death[4], [6].

Tumor-derived EVs are known to play a role in a number of tumor progression processes, such as metastasis and chemotherapy resistance[7]–[9].

Cianciaruso et al. developed a method to analyze tumor-associated macrophages’ secreted EVs[5].

Following the proposed method[5], tumor-derived EVs were isolated from breast cancer cells. They were then harvested and labelled with a fluorescent lipid.

After that, the tumor-derived EVs were added to a mouse macrophage culture and their uptake was monitored for 6 hours under Nanolive’s 3D Cell Explorer-fluo. While data regarding refractive index was obtained every 10 seconds, the cadence of fluorescence acquisitions was increased in order to avoid fluorescence-induced cell perturbations that may had compromised the observed phenomenon.

As observed in the footage, fluorescence intensity increases over time as EVs uptake increases.

Strikingly, EVs structures are visible using Nanolive’s imaging. They appear as small, dotty, membrane-dense structures appearing and growing at the center of the badly resolved fluorescent signal. Thanks to the high temporal resolution, fine dynamics of such process can be extracted.

 

[1]         S. Caruso and I. K. H. Poon, “Apoptotic cell-derived extracellular vesicles: More than just debris,” Frontiers in Immunology, vol. 9, no. JUN. Frontiers Media S.A., 28-Jun-2018.

[2]         M. Colombo, G. Raposo, and C. Théry, “Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles,” Annu. Rev. Cell Dev. Biol., vol. 30, no. 1, pp. 255–289, Oct. 2014.

[3]         M. Mathieu, L. Martin-Jaular, G. Lavieu, and C. Théry, “Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication,” Nature Cell Biology, vol. 21, no. 1. Nature Publishing Group, pp. 9–17, 01-Jan-2019.

[4]         G. Van Niel, G. D’Angelo, and G. Raposo, “Shedding light on the cell biology of extracellular vesicles,” Nature Reviews Molecular Cell Biology, vol. 19, no. 4. Nature Publishing Group, pp. 213–228, 01-Apr-2018.

[5]         C. Cianciaruso et al., “Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles,” Cell Rep., vol. 27, no. 10, pp. 3062-3080.e11, Jun. 2019.

[6]         M. Yáñez-Mó et al., “Biological properties of extracellular vesicles and their physiological functions,” Journal of Extracellular Vesicles, vol. 4, no. 2015. Co-Action Publishing, pp. 1–60, 2015.

[7]         C. F. Ruivo, B. Arbara Adem, M. Silva, S. Onia, and A. Melo, “The Biology of Cancer Exosomes: Insights and New Perspectives,” 2017.

[8]         I. Keklikoglou, C. Cianciaruso, E. Güç, … M. S.-N. cell, and  undefined 2019, “Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models,” nature.com.

[9]         A. Becker, B. K. Thakur, J. M. Weiss, H. S. Kim, H. Peinado, and D. Lyden, “Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis,” Cancer Cell, vol. 30, no. 6. Cell Press, pp. 836–848, 12-Dec-2016.

 

 

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